Role of MYC in Anchorage-Dependent Growth
Abstract
When deprived of adhesion normal cells are unable to proliferate; instead they arrest in G1 phase of the cell cycle. Unlike normal cells, transformed cells are able to by-pass this adhesion-mediated control of cell proliferation and grow in an anchorage-independent manner. Understanding the mechanism by which the extracellular matrix controls, progression through the G1 Phase of the cell cycle would give us insight into the cellular mechanism that accompany carcinogenesis. We have shown, here using a human mammary epithelial model, that the expression of the early growth control gene, c-Myc, is directly regulated by cell adhesion through a 1 integrin-dependent pathway, involving the PKC, c-Src family of kinase, and MAPK. When deprived of adhesion mammary epithelial cells are unable to progress into S phase upon EGF stimulation, and they arrest in early G1. This Anchorage-dependent block correlates with the down regulation of c-Myc mRNA and Protein.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2000
- Accession Number
- ADA391639
Entities
People
- Christelle M. Benaud
Organizations
- Georgetown University