Inhibiting Tumorigenesis by Growth Factor Receptor Down Regulation Using a Sorting Nexin
Abstract
Excessive activation of growth factor receptors can lead to the unrestrained cellular proliferation characteristic of tumors. Our objective is to determine if SNX1, a protein involved in intracellular membrane trafficking, can be used to downregulate EGF receptors in mammary gland. Our approach is to characterize the gene for SNX1 and to generate transgenic animals overexpressing SNX1 in mammary glands. We have characterized a genomic clone for SNX1 and had planned to use this clone for transgenic vector construction. However, the size of the first intron in SNX1 was too large for this approach to be used successfully. Instead, a WAP-SNX1 cDNA vector was been constructed and was used to generate transgenic animals. One transgenic positive mouse has been identified. A Career Development Award was a second component of the application. Career development activities include: participation as reviewer on the American Cancer Society Cell Structure and Metastasis study section and participation on a search committee charged with identifying a Director for Breast Cancer Research at UAMS. In addition, the State of Arkansas Breast Cancer Research Program awarded me a one-year pilot research grant to examine the relationship between HER-2/neu and EGF receptors in mammary gland cell proliferation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2001
- Accession Number
- ADA391920
Entities
People
- Richard Kurten
Organizations
- University of Arkansas for Medical Sciences