Quantifying the Effects of Preventative Food on the Metabolism of a Prostate Carcinogen in Humans and in Prostate Cells Grown in Culture
Abstract
We are investigating the effects of foods associated with reduced prostate cancer risk on a dietary carcinogen known to be associated with cooked meat and elevated cancer risk. Cooked muscle meats contain potent mutagens and carcinogens belonging to the heterocyclic amine class of compounds. One of these, PhlP, is a genotoxic carcinogen that has been shown to cause DNA damage in prostate tissue and prostate tumor formation in rats. We have developed a method to quantify urinary metabolites of PhlP in human volunteers that have been fed a meal of cooked chicken. Using this method, we have shown that broccoli may effect both the rate and relative amounts of PhlP metabolite excretion. At the cellular level we are investigating the metabolism of PhlP in human prostate cancer cells and have shown that PhlP,-OH-PhlP and sulforaphane, the putative active ingredient in broccoli, have an effect on cell growth. This research uses state-of-the- art analytical measurement methods to support conclusions about the role of diet and prostate cancer in humans. Although still preliminary, our results indicate that other components of the diet, such as broccoli, may have an effect on the metabolism of a commonly-occurring food carcinogen.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2001
- Accession Number
- ADA391955
Entities
People
- James S. Felton
Organizations
- Lawrence Livermore National Laboratory