Characterization of Tubulin Isoforms in Breast Cancer Cells
Abstract
Tubulin, the alpha beta dimeric protein of microtubules!, bind to different antitumor drugs which are routinely used for cancer chemotherapy. Both alpha- and Beta-tubulin exist as 7-S different isoforms which are expressed differently in different tissues, and also undergo various post-translational modifications including tyrosination-detyrosination, acetylation, poly-glutamylation, polyglycylation and phosphorylation. It is not known whether breast cancer cells differ in the tubulin isoform level or their post-translational modifications. The long-term goal of this project will be to find new prognostic markers for an early detection of breast cancer and to develop alternative therapies against breast cancer. The primary goal is to study the expression of different forms of tubulin and their post-translational modifications in human breast cancer cells. Our previous results showed that paclitaxel resistant breast cancer cells express BetaIII isoform selectively. Thus, it was felt necessary to make a full length cDNA of BetaIII isoform for overexpression in breast cancer cells. Here we report the preparation of the cDNA and its overexpression in breast cancer cells. Stable MCF-7 cell lines overexpressing Beta III isoform are found to be more resistant to paclitaxel when compared with the wild-type MCF- 7 cells
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2001
- Accession Number
- ADA392266
Entities
People
- Asok Banerjee
Organizations
- University of Texas Health Science Center at San Antonio