Metal Ion Complexes for Antibody Imaging and Therapy in Breast Cancer
Abstract
The goals of this project are to prepare and test novel bifunctional chelates based on DOT A to antibodies directed to CEA (carcinoembryonic antigen) and Her2/neu. The conjugates will be radiolabeled with In-111 for tumor imaging. and Y90 for tumor therapy. In the first year of the project we have shown that the CEA positive MCF7 cell line transfected with Her2/neu can be grown in nude mice injected with estrogen pellets and used as a tumor model. Since we have previously conjugated anti-CEA antibody T84.66 with DOTA and shown it to target CEA positive tumors, we began our work with the anti-Her2/neu antibody 4D5. In the second year of the project, we have extended these studies to the humanized version of 4D5, Herceptin, and shown that good tumor targeting can be achieved even for parental MCF7 cells that don't over-express Her2/neu. We have also shown that Y90-anti-Her2/neu therapy is about equally effective to Y90-anti-CEA therapy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2000
- Accession Number
- ADA392386
Entities
People
- John E. Shively