Celluar and Molecular Roles of the Akt Protein Kinase in Breast Carcinomas
Abstract
Akt is a serine/threonine kinase that has been implicated in the iitiation and/or progression of breast cancers. In order to gain an understanding of how Akt promotes malignant transformation, we identified proteins that are regulated by phosphorylation by Akt, including the B-Rafserine/threonine kinase and the Elk-1 transcription factor. Akt negatively regulates the enzymatic activity of B-Raf in vitro and in vivo by phosphorylating two residues in the amino-terminal regulatory domain of B-Raf. Akt also negatively regulates the activity and stability of the Elk-1 transcription factor. Thus, Akt may promote transformation and cell survival in part by altering gene expression through regulation of transcription factors by phosphorylation and in part by regulating the Ras/Raf pathway by phosphorylating and downregulating the activity of the B-Rafkinase.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2001
- Accession Number
- ADA392394
Entities
People
- Anne B. Vojtek
Organizations
- University of Michigan