A Novel Gene Gun-Mediated IL-12 Gene Therapy for Breast Cancer
Abstract
The results of this study show that particle-mediated IL-12 gene therapy was effective against mammary tumors in mouse models. IL-12 gene therapy of the immunogenic TS/A adenocarcinoma resulted in regression of the established primary tumors and induction of immunological memory. This IL-12 gene therapy protocol for the poorly immunogenic 4T1 adenocarcinoma did not significantly affect the growth of the primary tumor, but could reduce spontaneous metastasis into the lungs. The anti-metastatic effect of IL-12 was not mediated by T cells, but involved NK cells and inteiferon gamma. In combination with IL-12 gene therapy, IL-18 and ICE genes were found to be more effective in treatment of established TS/A mammary tumor than IL-12 alone. These results suggest that particle-mediated IL-12 gene therapy, alone or in combination with other immunological approaches, may be effective for treatment of breast cancer. In a separate, but strategically relevant approach for cancer gene immunotherapy, a strong T cell- mediated anti tumor effect was demonstrated in mice vaccinated with a gene encoding the tumor-associated antigen gplOO in combination with a GM-CSF gene. These results have served as the pre-clinical data for a first clinical trial using cutaneous particle-mediated gene transfer, initiated at the University of Wisconsin.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2000
- Accession Number
- ADA392504
Entities
People
- Ning-sun Yang