Characterization of SIRPs in Prostate Cancer Cells
Abstract
We have studied three prostate cancer lines for their ability to make proteins that alter the growth of cells. These proteins, called SIRPs' (signal regulatory proteins), are expressed on the surface of cells. There are two types of SIRPs. One inhibits cell growth, while the other enhances it. SIRPs also interact with proteins expressed on cells belonging to the immune system. Thus, SIRPs may regulate both the growth of prostate cancer cells and their interactions with the immune system. We first showed that prostate cancer cells express a gene for an inhibitory SIRP. We have now made new reagents (monoclonal antibodies), which demonstrate the expression of SIRP proteins on at least two of the three prostate cancers. By gene transfer, we have also expressed an inhibitory SIRP at high levels in one of the prostate cancer cell lines. This will allow us to study the effect of the SIRP on the response of the cancer cells to growth hormones, and to examine the effects of mutations in SIRPs. We are also defining the range of SIRP genes expressed in the prostate cancer cells. These were the goals of our proposal, and we expect to accomplish them all.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2001
- Accession Number
- ADA392515
Entities
People
- William E. Seaman
Organizations
- Northern California Institute for Research and Education