Procathepsin D Stimulation of Human Breast Cancer Cell Growth
Abstract
The long-term goal is to develop a new breast cancer treatment based on inhibition of the growth factor activity of procathepsin D. During the current year we tested all possible breast cancer cell lines for secretion of procathepsin D. Based on our data, we prepared numerous clones of MDA-MB-23l cells transfected with all expected vectors. Both the secretion of procathepsin D in vitro and growth activity in vivo of these clones has been established. A library of 10 synthetic peptides with a single substitution of one amino acid have been prepared and tested. Based on this information we know the exact binding moiety on the molecule of activation peptide. We also prepared monoclonal antibodies against these peptides and tested them as potential inhibitors of breast cancer cell growth.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2001
- Accession Number
- ADA392652
Entities
People
- Vaclav Vatvicka
Organizations
- University of Louisville