Isolation of Motile Tumor Cells From Live Breast Tumors
Abstract
During metastasis from the primary tumor, cell motility is believed to have an important role in the dissemination of tumor cells. Isolating the motile population of cells from a tumor allows for a unique way of studying the mechanisms of cell motility involved in intravasation. We have developed a matrigel filled catheter that when placed into a tumor mimics the extracellular and vessel environments found in tumors. With this microneedle catheter we are able to selectively collect up to 1000 cells in needles that contain 25 nM EGF or 50,000 units/ml of CSF-1. By amplifying the RNA isolated from these cells we will be able to use microarrays to do gene discovery to find gene products that are associated with cell metastasis and motility. These gene products then can be used as targets for clinical and pharmaceutical research to further understand and prevent metastasis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2001
- Accession Number
- ADA393093
Entities
People
- John Condeelis
Organizations
- Albert Einstein College of Medicine