Nutritional Effect on Androgen-Response Gene Expression and Prostate Tumor Growth
Abstract
The objective of our research is to understand the molecular mechanism underlying the impact of various dietary components including fat, genistein, vitamin D, and selenium on prostate growth. Our research has demonstrated that the ventral prostate of the rats on high fat diet is 15% (p<O.OO1) heavier than that of the rats on low fat diet. The dietary influence on ventral prostate weight does not seem to involve androgen action axis because dietary components did not influence the expression of several androgen-response genes, serum testosterone (T) and dihydrotestosterone (DHT), and intraprostatic T and DHT in experimental animals. Thus, high fat diet is likely to modulate the ventral prostate weight via an androgen-independent mechanism. Using a highly sensitive PCR-based cDNA subtraction method, we have identified one gene that is down-regulated by dietary fat in the ventral prostate. Sequencing analysis revealed that this gene encodes prostatein C3, a ventral prostate-specific secretory protein. Dietary fat down-regulates the expression of prostatein C3 by 37% (p<O.O5). Prostatein C3 represents the first example of genes that are regulated by dietary fat in the prostate in vivo. Our findings are potentially important because dietary fat is a major risk factor associated with prostate cancer incidence.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2001
- Accession Number
- ADA393146
Entities
People
- Zhou Wang
Organizations
- Northwestern University