Pain Transmission in Humans: The Role of Novel Sensory Ion Channels
Abstract
The primary accomplishments of the previous funding period were: 1) cloning and expression of the mouse isoform of Scn10a gene product (SNS; TTX-resistant sodium channel); 2) functional heterologous expression and initial biophysical characterization of the mouse Scn10a gene product in sympathetic neurons; 3) cloning and sequencing of 4 Kbp of upstream genomic DNA; and 4) identification of BAC (bacterial artificial chromosome) clones harboring the mouse Scn10a gene. The nucleotide sequence of the mouse Scn10a cDNA obtained in these studies deviates significantly (170 discrepancies in 5824 bases) from a previously published cDNA sequence derived from genomic DNA (GenBank accession number Y09l08) and thus represents new information. Heterologous expression of the Scn10a cDNA in sympathetic neurons revealed a significant depolarizing shift in the biophysical properties of the expressed channel when compared with natively expressed channels.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2001
- Accession Number
- ADA393457
Entities
People
- Stephen R. Ikeda