Multiparametric Evaluation of Marker Expression

Abstract

During first year of this project, we have carried out multiparametric flow analysis of estrogen receptor (ER) expression in 75 formalin fixed/paraffin embedded human breast tumors. Flow cytometric data was compared with immunohistochemical studies and correlation between ER expression and the histopathological evaluation was undertaken. A manuscript is being prepared for public an on We have developed a high-resolution flow cytometer for measurement of nuclear volume and DNA content of nuclei. We developed a staining protocol for simultaneous evaluation of nuclear volume and DNA content of nuclei. A manuscript was published. We have analyzed 50 fresh/frozen tumor biopsies for nuclear volume versus DNA content and a manuscript is being prepared for publication.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2001
Accession Number
ADA394208

Entities

People

  • Awtar Ganju-krishan

Organizations

  • University of Miami

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Cancer
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Health Services
  • High Resolution
  • Measurement
  • Medical Personnel
  • Neoplasms
  • Oncology
  • Three Dimensional

Readers

  • Breast cancer cell signaling and growth regulation.
  • Business Analytics
  • Oncology and Biomarker-Based Cancer Detection.