A Novel Strategy to Isolate Invasion-Inducing Proteins from Human Breast Tumors
Abstract
This proposal describes the development of a screening strategy that can be applied to the identification of genes that regulate the progression of breast tumors to a metastatic state. The general approach is to make expressible cDNA libraries from highly invasive breast tumor cell lines, and to transfer these libraries (and the phenotype) to human tumor cells that normally do not exhibit invasive properties. This report covers the first 10 months of this project and describes: (1) the construction and characterization of 6 retrovirus-based expression libraries, (2) the efficient conversion of plasmid-based libraries into libraries of high titre infectious retroviral particles, and (3) a preliminary test screen of one of the libraries. A population of 250,000 infected cells has been passed through three rounds of enrichment and analyzed for the presence of proviral inserts. A large, but manageable number of inserts have been identified by PCR and are now being recovered for further analysis. To summarize, the screening is progressing according to our original schedule, and we anticipate that some of the proviral inserts that we are currently recovering will exhibit invasive potential.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2001
- Accession Number
- ADA395570
Entities
People
- Ian P. Whitehead