Prevention of Breast Cancer in IGFBP
Abstract
In this proposal, we hypothesize that inhibition of IGF action by IGFBP-1 will prevent breast cancer in a SV40 Tag transgenic model of breast cancer. We will test this hypothesis with two specific aims: 1) to inhibit-IGF action at the mammary epithelial cell by creating transgenic mice that express IGFBP-1 under the control of the whey acidic protein (WAP) promoter and 2) to test the ability of IGFBP-1 to suppress tumorigenesis by mating these animals with C3/Tag transgenic mice. To achieve these goals, we have created the expression vector, injected the construct into mice, and now have our first generation of mice. Of the animals we have analyzed, approximately 25% have the transgene. We are currently in the process of mating the F1 generation and determining if we have achieved IGFBP-1 expression in the mammary gland.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2001
- Accession Number
- ADA396151
Entities
People
- Douglas A. Yee
Organizations
- University of Minnesota