Enhancing Biological Detection With Dendrimer-Based Immunosensors

Abstract

Dendrimers are highly branched, tree-like polymers that possess precise size and shape, as well as surface groups. Among them, polyarnidoamine dendrimers are the most widely studied water-soluble/biocompatible dendritic macromolecules. Such dendrimers have been exploited for interesting applications in immunodiagnostics, magnetic resonance imaging, gene therapy, and drug delivery. This report introduces a new signal amplification strategy using dendrimers. While only one fluorescein group can be linked onto an antibody molecule, a large number of fluorophores can be attached to an antibody through a dendrimer linker molecule, thus forming a water-soluble fluorescein-dendrimer-antibody bioconjugate. Upon addition of the antigen, enhanced fluorescence signals are obtained when compared with the corresponding fluorescein-antibody analogs.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2001
Accession Number
ADA396321

Entities

People

  • Amanda L. Jenkins
  • Kate K. Ong
  • Ray Yin

Organizations

  • United States Army Research Laboratory

Tags

DTIC Thesaurus Topics

  • Amplification
  • Antibodies
  • Biological Detection
  • Detection
  • Dyes
  • Fluorescence
  • Fluorophores
  • Gene Therapy
  • Immunosensors
  • Laser Dyes
  • Luminosity
  • Macromolecules
  • Magnetic Resonance
  • Magnetic Resonance Imaging
  • Military Research
  • Molecules
  • Polymers

Readers

  • Molecular and Cellular Biochemistry
  • Nanocomposite Materials Science

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech