A Search for Mutations that Affect Susceptibility to Breast Cancer
Abstract
The purpose of this project is to search for mutations that affect breast cancer by screening the progeny of mice that were injected with ethylnitrosourea (ENU). ENU-treated males will be mated with MN-lO partners that carry the c-neu oncogene under the control of the mouse mammary tumor virus (MMTV) promoter, and the female progeny will be examined for breast cancer susceptibility. The scope will be limited to those mutations that occur in 180 ENU-treated males, and which interact with the c-neu oncogene. The major findings to date are related to the methods needed for treating this strain of mice with the highly toxic mutagen, ENU. We experienced some difficulties with mortality when we did the first injections, although we followed published procedures precisely. These problems have now been solved, and 86% of the mice treated by this procedure are still living. We now have 61 of the 180 EWU-treated mice that were originally proposed, and are waiting for them to recover their fertility. Their female progeny will be observed for their susceptibility to breast cancer, and in those lines which there is an increase or decrease relative to the control MN-lO line, the mutant gene will be studied by genetic mapping. The overall significance of this is that it will be possible to identify the corresponding human gene.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2001
- Accession Number
- ADA396342
Entities
People
- David L. Gasser
Organizations
- University of Pennsylvania