Characterization of a Putative Tumor Suppressor in Breast Cancer

Abstract

The RASSFlA locus is epigenetically inactivated in most lung and breast tumors. Expression of RASSFlA is sufficient to revert the growth transformation of human tumor cell lines. We show here that RASSFlA engages the Rb-family cell cycle checkpoint by inhibiting accumulation of cyclin Dl protein. Rare alleles of RASSFlA, isolated from tumor cell lines, encode proteins with alterations that block phosphorylation of RASSFlA on a putative ATM kinase phosphorylation site. These RASSFlA variants cannot inhibit proliferation of tumor cell lines, and suggest that RASSFlA activity is regulated by protein kinase cascades.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2001
Accession Number
ADA396348

Entities

People

  • Latha Shivakumar

Organizations

  • University of Texas at Dallas

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Carcinoma
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemical Reactions
  • Chemical Synthesis
  • Chemistry
  • Chromosomes
  • Genetic Code
  • Genetic Structures
  • Genetics
  • Health Services
  • Oncology
  • Proteins
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics