Identification of Structural Domains of ESX Required for Breast Cell Transformation
Abstract
The ESX gene is an Ets member that is potentially very important in breast cancer because the ESX genomic region (chromosome 1q32.1) is amplified in 50% of early breast cancers and ESX mRNA is over-expressed in human breast ductal carcinoma in situ (DCIS). However, the identity, ability and precise molecular mechanism by which any given Ets factor mediates breast cell transformation all remain unknown. The data that we have generated thus far show that ESX, a putative downstream effector of the HER2/neu pathway, regulates expression of the HER2/neu promoter and mediates transformation of MCF-12A human breast cells. Moreover, using the anti-ESX antibody that we generated in collaboration with ABR, Inc, we have shown that the non-transformed MCF-12A cells fail to express ESX, while the transformed and malignant T47D breast cancer cells express abundant amounts of ESX. When we enforce ESX expression in MCF-12A cells, they display increased adhesion, migration and invasion.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2001
- Accession Number
- ADA396355
Entities
People
- Arthur Gutierrez-hartmann
Organizations
- University of Colorado Health