Analysis of the TACC1 Gene from the 8p11 Chromosome Region Frequently Amplified in Metastasizing Breast Cancer
Abstract
The TACC1 gene was isolated from a region of chromosome 8pll which shows amplification in breast cancers, especially those which show metastasis to the lymph nodes. Preliminary studies showed that overexpression of TACCl in mouse fibroblasts cause morphological transformation. We have now shown, using immunohistochemistry and immunoprecipitation, that TACCl is present in the cytoplasm and the nucleus and furthermore that it probably associates with the mitotic spindle microtubules. Yeast two hybrid analysis has identified a series of genes which interact with TACCl. Two of these, SIAHl and GAS4l, appear to be associated with growth regulatory pathways which may implicate TACCl in cell growth control and hence why overexpression leads to progression of breast tumors. Two other proteins, pl6 and L-Sm7, also interact with TACC1 and these proteins are apparently involved in the transportation of proteins to the nucleus. Thus it appears that TACC1 Makes important associations in the cytoplasm which then facilitate the transportation of TACC1 and probably the whole protein complex into the nucleus where it may affect mitosis. Further characterization of the intracellular pathways which use TACC1 for their function will help further understand its role in breast tumorigenesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2001
- Accession Number
- ADA396393
Entities
People
- John K. Cowell