Pim-1, A Potential Participant in Breast Cancer
Abstract
This year I have completed preliminary work on the project, "Pim-1, a potential participant in breast cancer". All work done to date has concentrated on those experiments outlined in Task 1 of the "Statement of Work". Initial studies have been completed showing by Western blot that Pim-1 protein expression can be induced by prolactin, as well as with dexamethasone and insulin which are often used together with prolactin when looking at genes regulated by this lactogen. This work needs to be completed with repeated experiments using the proper controls. I have recently contacted Dr. Wolfgang Doppler (University of Innsbruck, Austria) for the human beta-casine in promoter-CAT construct and he has agreed to send this to me for use as a control in these and other experiments. Since I am looking at a system where there are site-specific interactions between proteins and DNA and there are slight variations in protein and DNA sequence between species, I feel it is important to use all human components, such as human beta-casein constructs and human beta-casein constructs. However Dr. Doppler has also recommended that I use the rat beta-casein promoter reporter construct and I have requested this as well.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2001
- Accession Number
- ADA396396
Entities
People
- Maria Meyer
- Nancy S. Magnuson
Organizations
- Washington State University