Microarray Technology to Study the Role of Genetic Polymorphisms in Breast Cancer Risk
Abstract
It has been suggested that low penetrant alleles are associated with breast cancer risk. Although the contribution of low penetrant alleles to the individual risk is relatively small, they can contribute to a large proportion of breast cancer cases in the population. In this study we took the candidate gene approach to study the association of 32 different genetic polymorphisms in a population-based sample. We have also proposed to use the power of high-throughput microarray technology to identify low penetrant genetic polymorphisms which contributed to the risk of developing breast cancer. To date, we have successfully accomplished several goals in the development of this technology. Using the PCR primers designed for each polymorphism, a panel of cell lines has been screened using SSCP/Sequencing and the control DNA specimens corresponding to different alleles were determined. Allele-specific oligonucleotides for the purpose of probe preparation were designed for each allele, and using the control DNA samples and the PCR strategy their specificity were evaluated.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2001
- Accession Number
- ADA396472
Entities
People
- Hilmi Ozcelik
Organizations
- Mount Sinai Hospital, Toronto