Molecular Mechanisms of Calcium Hydroxyapatite Crystal Induced Nitrogenesis in Breast Cancer

Abstract

For some time microcalcifications associated with breast lesions were considered to represent an epiphomenon, useful only as a radiological indicator of breast carcinoma. However, the data presented here shows that HA crystals are capable of exerting significant biological effects on surrounding cells. In this study we report that HA increases mitogenesis in both normal and malignant mammary cell lines. Particles of latex beads, of similar size and concentration to HA crystals, had no effect on mitogenesis. We have also shown that HA crystals stimulate mitogenesis of quiescent mammary cell lines in a concentration dependent fashion. Our results suggest that exposure to HA crystals can cause a significant increase in PGE2 possibly by induction of COX, which appears to be a critical factor in regulating the mitogenesis of those cells. These findings support our hypothesis that HA crystals may contribute to breast cancer progression by amplifying the pathological processes surrounding a lesion and underlie the importance of further studies of the pathological potential of microcalcifications consisting of HA crystals in breast oncology.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2001
Accession Number
ADA396494

Entities

People

  • Geraldine Mccarthy
  • Maria A. Morgan

Organizations

  • Royal College of Surgeons in Ireland

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Calcium Compounds
  • Cancer
  • Carcinoma
  • Cell Count
  • Cell Line
  • Cells
  • Chemistry
  • Culture Techniques
  • Epithelial Cells
  • Health Services
  • Neoplasms
  • Oncology
  • Proteins
  • Statistical Analysis
  • Tumor Cell Line

Readers

  • Immunology
  • Immunology and Pathology
  • Oncology and Biomarker-Based Cancer Detection.