In-Vitro Cytotoxicity Screening of Plant Extracts
Abstract
The current year of activity included two main projects. The CEM/TART/1A2 anti-HIV screen was established to enable in-house evaluation of ICBG extracts for anti-HIV activity. This is a whole virus replication assay that follows HIV induced killing of human lymphocytes. HIV 1 tat(-)rev(-) was grown and stocks prepared for cryogenic storage. TART and 1A2 cell line growth was quantified in the absence and presence of HIV infection. The cytoprotection protocol developed by Kiser and colleagues at NCI was evaluated using AZT as a positive control. The assay was found to be too variable and inconsistent for our intended use. Alternative protocols were developed. Trypan blue exclusion was found to be a sensitive and robust measure of cell viability during HIV infection. Its advantage is its simplicity, requiring only a microscope, a hemocytometer and a counter. Its disadvantage is that it is labor intensive. In addition to development of the HIV assay, this year's activity evaluated fractions of cytotoxic extracts with anti-cancer potential. Extract 799 displayed minor DNA cleavage activity in whole cells, and powerful cell cycle effects. Fractions 1986 and 1999 were found to be the most cytotoxic fractions and 1986 was found to have in vitro activity after incubation with purified human topoisomerase II.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2001
- Accession Number
- ADA396523
Entities
People
- Louis R. Barrows
Organizations
- University of Utah