The Role of S100A7/RANBPM Interaction in Human Breast Cancer
Abstract
We previously identified the psoriasin (S100A7) gene as being differentially expressed between different stages of human breast cancer. High psoriasin expression was seen in DCIS compared to normal and invasive breast tissue. Protein expression was also found to be present in both the nucleus and the cytoplasm. Currently, there are no data on the biological role of psoriasin, and how its altered expression contributes to the biological process of invasion. To identify potential pathways or cellular processes in which psoriasin participates, we have utilized the yeast two-hybrid assay to identify interacting proteins. Full-length psoriasin was used to screen 17.3 x 106 clones from a normal human mammary cDNA library. Two centrosomal proteins, ranBPM and hGCP3, were determined to be true positives in the yeast assay and selected for further study. Co-immunoprecipitation studies confirmed the interaction between psoriasin and ranBPM. As well, confocal microscopy analyses show that psoriasin does indeed localize to the centrosome in two different human breast cancer cell lines that have been stably transfected with the psoriasin gene. Quantitative RT-PCR of selected human breast tumors was preformed to determine the level of psoriasin and ranBPM expression in relation to clinicopathological parameters. The biological importance of the psoriasin-ranBPM interaction is under investigation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2001
- Accession Number
- ADA396524
Entities
People
- Ethan D. Emberley
- Peter H. Watson
Organizations
- University of Manitoba