Isolation of Genes from Chromosome Region Ip31 Involved in the Development of Breast Cancer
Abstract
The p31 region of chromosome 1 shows frequent (50%) loss of heterozygosity (LOH) in breast tumors. This observation implies the presence of a tumor suppressor gene in this region which, when mutated contributes to tumorigenesis. The maximal common region undergoing LOH extends over approximately 2Mbp. We have constructed a map of overlapping BAC clones spanning this region with only 4 gaps. The total available sequence from this region suggests that approximately 50-60% of it is now completed. Using gene analysis tools, we have been able to demonstrate that few full-length genes are located in this region and that the ESTs from the databases are clustered to a proximal position of the contig. The high level of sequencing completed from this region means that it will soon be possible to establish a baseline transcription map. The genes identified in this way can now be tested systematically for mutations in breast tumors. The progesterone receptor gene lies at the limit of the region suggesting that it was possibly the target for LOH. We established the exon/intron structure of this gene and undertook SSCP analysis of breast tumors. No gene-inactivating mutations were found excluding this gene from involvement in breast cancer development.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2001
- Accession Number
- ADA396633
Entities
People
- John K. Cowell