Mechanisms of Intraductal Tumor Spread
Abstract
We have developed a three-dimensional computer-based quantitative microscopy platform to be applied to simultaneous morphological and molecular studies of both normal and neoplastic mammary tissue. Using our system, we can reconstruct relevant microscopic tissue structures (e.g. mammary ducts, lobules, lymph nodes, tumor masses, etc.) from consecutive thin tissue sections. Then, using 3D virtual reconstruction, we can perform quantitative morphological measurements combined with directed structure defined high resolution analysis of molecular events. The system will be applied to study the mechanisms of intraductal tumor spread, by reconstructing in 3D and performing cell-level genetic analysis of pre-invasive tumors. We'll look for morphologically normal, genetically abnormal cells within the normal ducts spreading from the primary tumor, which could explain the pre-metastatic spread of the disease.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2001
- Accession Number
- ADA396668
Entities
People
- Carlos O. De Solorzano
Organizations
- University of California, Berkeley