Identification of Oncogenes Cooperating in Murine Mammary Tumorigenesis

Abstract

In order to identify and characterize additional novel or unexpected proto-oncogenes that, in addition to fibroblast growth factors (Fgfs) cooperate with Wnt1 in murine mammary tumorigenesis, we have generated MMTV-infected Wnt1/0b/DN-Fgfr2 bitransgenic mice. While the Wnt oncogenic signal is constitutively overexpressed in their mammary gland, cooperative oncogenic Fgf signals should be abolished by the expression of a dominant-negative FGF receptor (DN-Fgfr2). Only those cells carrying MMTV-insertionally activated cellular proto-oncogenes, other than Wnts and Fgfs, should have a growth advantage in the bitransgenic mammary gland. The clonal expansion of these cells leads to mammary tumorigenesis. As proposed, we have generated a cohort of 25 MMTV-infected Wnt10b/DN-Fgfr2 bitransgenic females. As controls, we have also generated an uninfected bitransgenic cohort, as well as MMTV- infected/uninfected Wnt0b and DN-Fgfr2 female groups. To date, several mammary adenocarcinomas have appeared in the MMTV infected bitransgenic animals.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2001
Accession Number
ADA396713

Entities

People

  • Gregory M. Shackleford
  • Rocio S. Lopez-diego

Organizations

  • Children's Hospital Los Angeles

Tags

DTIC Thesaurus Topics

  • Abstracts
  • B Lymphocytes
  • Biomedical Research
  • California
  • Cells
  • Demographic Cohorts
  • Epithelial Cells
  • Glands
  • Growth Factors
  • Health Services
  • Identification
  • Infection
  • Lymphocytes
  • Mammary Glands
  • Proviruses
  • Viruses
  • Wound Infections

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics