Rolling Circle Transcription of Ribozymes Targeted to ras and mdr-1
Abstract
We report new progress on the use of the rolling circle transcription strategy for production of ribozymes in vitro and in living cells. Optimized circular DNA vectors have been selected from randomized libraries and the selected sequences were found to be transcribed significantly more strongly than the library as a whole by two different RNA polymerases. Optimized rolling circle vectors were then constructed to encode a ribozyme targeted to a drug resistance gene in a bacterium. The vector was then shown to lower the activity of this gene in the living bacterial cells. A similar strategy may one day be useful for inhibiting genes (such as breast cancer-associated genes) in human cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2000
- Accession Number
- ADA396819
Entities
People
- Eric T. Kool
Organizations
- University of Rochester