Recombinant Polyclonal Antibody Libraries for Breast Cancer Therapy
Abstract
This project proposes the development of a recombinant polyclonal antibody library specific for the human breast carcinoma cell line BT-20. The proposed library would target multiple epitopes on the human cell line and also provide the genes necessary for the unlimited perpetuation of the defined antibody mixture. Ultimately, such antibody libraries could be used for treatment and diagnosis of breast cancer in humans. In the present study, an Fab phage display library specific for the BT-2O cells has been generated. To obtain a polyclonal antibody library of whole IgG antibodies that would mediate effector functions, a vector system for mammalian expression has been constructed. The new system uses restriction enzyme-based transfer of variable region gene pairs from a phage display to a mammalian expression vector. This system will be used to complete the construction of an anti-breast cancer polyclonal antibody library.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2000
- Accession Number
- ADA396829
Entities
People
- Jacqueline Sharon
Organizations
- Boston University