HET is a Novel Tumor Suppressor Gene in Human Breast Cancer
Abstract
We have evidence that the nuclear matrix protein RET might represent an important tumor suppressor gene in human breast cancer: (1) Overexpression of HET inhibited growth. (2) it was negatively associated with S-phase fraction in breast tumors, and 16% of breast tumors did not express HET. (3) Western Blot analysis in breast tumors led to the detection of smaller products, presumably representing truncated HET proteins. (4) RET maps to a locus on chromosome 19P13 where we detected an unusually high rate of loss of heterozygozity. In the first specific aim we will directly answer whether RET is the tumor suppressor gene by performing additional LOH analysis and mutational analysis of RET in breast cancer cell lines as well as in tumors. in the second specific aim we will perform functional analysis of discovered RET mutations in breast cancer cell lines. if RET is the tumor suppressor gene at l9p13, this will have a direct impact on the mechanistic understanding of tumor suppressor genes in breast cancer, as this region has an extremely high LOH rate in human breast cancer. if our hypothesis is true, then mutational analysis of RET could become a very informative tool for breast cancer prognosis and therapy..
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2000
- Accession Number
- ADA396830
Entities
People
- Steffi Oesterreich
Organizations
- Baylor College of Medicine