Vitronectin and Integrin (alpha)v(beta)3 in Ovarian Carcinoma
Abstract
One hallmark of ovarian cancer is the presence of large amount of floating cells in ascites. However, it is unknown how ovarian cancer cells can survive in absence of adhesion. Our early studies showed that ovarian cancer cells express vitronectin and alphavbeta3 integrin on cell surface, and the interaction between them is essential for ovarian cancer cell survival in suspension. In present study, we found that engaging alphavbeta3 with vitronectin induced NF-kB activation; Super electrophoresis mobility shift assay with antibodies against individual member of NF-kB family showed that p5O and p65 were in the DNA-protein complex. To determine the importance of NF-kB activity in ovarian cancer cell survival, we blocked NF-KB activity by either expressing dominant negative form of IkB or using NF-kB inhibitors D6O9 or SN5O. In a 2-day growth period, the inhibition of NP- KB activity resulted in over 80% of cell death in ovarian cancer cells cultured in suspension, but did not significantly affect the survival of ovarian cancer cells cultured in adhesion. These results strongly suggest that vitronectin/ alphavbeta3 interaction-mediated NF-KB activity is essential for ovarian cancer cell survival in absence of adhesion, and may explain why ovarian cancer cells can survive in suspension.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2001
- Accession Number
- ADA396906
Entities
People
- Shuang Huang
Organizations
- Scripps Research