Androgen Regulation of Human Prostate Cell Growth
Abstract
Although treatment of prostate cancer by androgen withdrawal has been an established treatment for decades, the molecular basis for androgen-dependent prostate growth is unclear. Since androgen effects are mediated through changes in gene expression, the molecular basis for prostate cancer regression upon androgen withdrawal and eventual escape from withdrawal would be better understood if the genes regulated by androgen in the prostate were known. This laboratory had developed a method of identifying hormone-regulated genes by "trapping" (Harrison & Miller, Endocrinology 137:2758, 1996), using a selectable transgene lacking a functional promoter. After stable transfection, a two-stage selection is performed and only cells containing a transgene that has usurped the functions of a native, regulated promoter are left alive. The flanking, native DNA is then sequenced by gene-walking (Harrison et al, Biotechniques. 22:650-3, 1997). Over 100 LNCXAP clones have been isolated of which 80 contain trapped, down-regulated genes and 30 contain trapped, up-regulated genes. Initial walks have been performed on a total 20 DNA samples of which one is homologous to a gene not previously described as androgen-regulated. This gene has homology with known protein synthesis initiation factors and thus, may play a supporting role in androgen stimulation of prostate protein synthesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2001
- Accession Number
- ADA396909
Entities
People
- Robert W. Harrison Iii
Organizations
- University of Rochester