Regulatory Control of Breast Tumor Cell Poly (ADP-Ribose) Polymerase
Abstract
Poly (ADP-ribose) polymerase (PARP) is a component of the DNA synthetic apparatus of cells, (i.e., the DNA synthesome). PARP is involved in the process of DNA replication, DNA repair, and cellular transformation. At least 15 components of the DNA synthesome are poly (ADP-ribosylated), which suggests that PARP may play a regulatory role in controlling the activity and the fidelity of the DNA synthesome of breast cancer cells. We also observed that the level of PARP activity found in breast cancer cells is below that found in non-malignant breast cells. We have also confirmed that PARP is a component of the DNA synthesome by both MALDI and quadrapole-time of flight (Q-TOF) mass spectrometry. We also observed PARP to be more highly expressed in the breast cancer cells, despite a lower overall activity. It is our goal to establish a clear link between the differences in the activity of PARP and the alteration(s) in structure exhibited by this protein in both malignant and non-malignant breast cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2001
- Accession Number
- ADA397014
Entities
People
- Linda Malkas
- Suhua Han
Organizations
- University of Maryland, Baltimore