Blocking HER-2-Mediated Transformation with a Dominant Negative Form of HER-3

Abstract

Amplification of the HER-2 gene often leads to breast cancer by causing cells to make abnormally high levels of the wild-type HER-2 protein. Evidence now shows that the interaction between HER-2 and HER-3 leads to the constitutive activation of HER-2/HER-3 heterodimers in breast cancer cells with HER-2 gene amplification, and HER-2/HER-3 potently activates multiple signal transduction pathways involved in mitogenesis. This indicates that inhibition of the interaction between HER-2 and HER-3 may be an especially effective and unique strategy for blocking the effects of HER-2 in human breast cancer cells. Therefore, we constructed a bicistronic retroviral expression vector that codes for a dominant negative form of HER-3 that can inactivate the function of HER-2/HER-3.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2001
Accession Number
ADA397093

Entities

People

  • Tracy G. Ram

Organizations

  • Washington State University

Tags

DTIC Thesaurus Topics

  • Albumins
  • Amplification
  • Biological Factors
  • Biological Sciences
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Culture Techniques
  • Epithelial Cells
  • Gene Therapy
  • Growth Factors
  • Inhibition
  • Molecules
  • Neoplasms
  • Proteins

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.