The Optical Patch Clamp. Stage III. Single Channel Activity by Ultra-Resolution Optics

Abstract

This award has resulted in 3 major achievements: (1) Second harmonic imaging microscopy (SHIM), a new modality for non-linear optical microscopy, SHIM, has been developed and shown to enable 3D imaging of thick specimens with sub-micrometer resolution. (2) Enhancement of second harmonic generation (SHG) signals by gold nanoparticles has been demonstrated and shows great potential for functional mapping of biological surfaces at the single molecule level. (3) A series of new dyes have been synthesized that have uniquely advantageous properties for both SHG and protein labeling applications.

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Document Details

Document Type
Technical Report
Publication Date
Dec 26, 2001
Accession Number
ADA397457

Entities

People

  • Leslie M. Loew

Organizations

  • University of Connecticut Health Center

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Cells
  • Chemical Synthesis
  • Chemistry
  • Demographic Cohorts
  • Detection
  • Detectors
  • Electrophysiological Phenomena
  • Fluorescence
  • High Resolution
  • Membrane Potentials
  • Metallic Nanoparticles
  • Micrometers
  • Molecules
  • Nanoparticles
  • Optical Phenomena
  • Second Harmonic Generation
  • Skeletal Muscle

Readers

  • Materials Science and Engineering.
  • Nanoscale Plasmonic Nanotechnology

Technology Areas

  • Biotechnology