Factors That Affect Fluorescein Analysis
Abstract
Quality assurance aspects are of considerable consequence in experimental studies. Because of the widespread use of fluorescein as an analytical tracer in aerosol studies, a set of experiments was conducted that summarize the effects of parameters that influence its detection and recovery from filters. The results confirmed the following: (1) the optimum excitation and emission wavelengths for fluorescein are 492 and 516 nm, respectively; (2) the fluorescence intensity from a fluorescein solution is strongly pH dependent; (3) pH does not affect the shape or the position of the peak of the emission spectrum; (4) changes in solution temperature cause a change in fluorescent intensity; (5) at concentrations below the self-quenching limit, fluorometer readings increase in direct proportion to fluorescein concentration; (6) the recovery procedure of fluorescein from glass fiber filters is quantitative; (7) fluorescein samples, prepared for fluorometer measurements, appear to be stable over at least a 12-day period; and (8) at higher gain settings, use of different test tubes and changes in test tube orientation can affect the fluorescence intensity measurement of low fluorescence material. As an example, the procedures are applied to determine aerosol penetration through two sample transport lines.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2001
- Accession Number
- ADA397677
Entities
People
- Andrew Mcfarland
- Daniel G. Wise
- Jana Kesavan
- Robert W. Doherty
Organizations
- Texas A&M University