Are Microtubules Involved in Anoikis
Abstract
This project is investigating the role of microtubule alterations in anoikis, with a view toward re-examining the mechanism of microtubule-targeting drugs such as taxol. During the past year, we have uncovered a new mechanism by which cell adhesion controls apoptosis. The death receptor adaptor protein FADD (FAS-associated death domain protein) is critical for anoikis as well as death ligand (e.g., FASL) - induced apoptosis. Recently, we discovered that FADD is primarily in the nucleus of attached cells, where it is unavailable for apoptosis induction. Detachment of mammary epithelial cells from extracellular matrix, however, provokes the export of FADD from the nucleus, thus promoting apoptosis. Our preliminary work suggests that microtubule drugs can promote FADD export, suggesting a new mechanism by which they can promote apoptosis. This is expected to have major ramifications for optimizing the use of taxol or other microtubule drugs in connection with other agents that may promote apoptosis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2001
- Accession Number
- ADA397720
Entities
People
- Steven M. Frisch
Organizations
- Sanford Burnham Prebys Medical Discovery Institute