Reactive Oxygen is a Major Factor Regulating Cell Division and Angiogenesis in Breast Cancer
Abstract
We investigated the occurrence, origin and have begun to explore the biological function of elevated reactive oxygen species (ROS) in breast carcinoma cell lines, particularly with regard to aberrant expression of Nox isoforms. We have initially surveyed 7 breast carcinoma cell lines including BT-20, BT-474, MCF7, MDA-MB-231, SK-BR-3, T-47D, and ZR-75-1, available through ATCC. These include lines developed from both primary and metastatic tumors. In addition, we surveyed three control cells lines, MCFlOA, MCFl2A, and 184A1 derived from either fibrocystic disease or breast reduction. We investigated the generation of the H2O2 and O2%. While O2 levels appeared to remain unchanged, 11202 levels increased significantly over control cell lines in several of the tumor cell lines. Interestingly, the addition of estradiol to the estradiol dependent MCF7 cell line greatly increased 11202 production. Increased 11202 levels corresponded with increased tumorigenicity based on literature searches. Analysis of mRNA expression of the Nox isoforms revealed that the above cell lines contain the message for Nox4 and Nox5. We have recently tested the cell lines for Nox4 and Nox5 protein expression using Nox4 and Nox5 antibodies developed in our lab. Nox4 expression is very apparent in the cells. Nox5 protein expression needs further exploration.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2001
- Accession Number
- ADA397762
Entities
People
- Rebecca S. Arnold
Organizations
- Emory University