Role of the Adherens Junction Protein Fascin in the Regulation of Tight Junction Permeability in the Mouse Mammary Gland
Abstract
An adenovirus based gene delivery system was developed which allows the delivery of target genes to the mouse mammary gland epithelium and to cultured mouse mammary epithelial cells. Transduction of the mammary gland does not induce inflammation or effect tight junction permeability. Transduced cells are morphologically normal and produce milk. This gene delivery system was used to express an N-terminally truncated mutant of the tight junction protein occluding in the mammary gland and in cultured cells. Transgene expression caused programmed epithelial cell death (anoikis) in the mammary gland and in cultured cells. Truncated occludin localized to the tight junction 18 hours post transduction and showed vesicular distribution 48 hours post transduction. The tight junction protein ZO-1 left the tight junction and colocalized with the transgene. The apical junction complex, actin bundling protein fascin was up-regulated and diffusely distributed in transduced cells. The fascin binding, adherens junction protein beta-catenin was distributed to the perinuclear region of transduced cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2001
- Accession Number
- ADA397802
Entities
People
- Margaret Neville
- Neal E. Beeman
Organizations
- University of Colorado Health