P16 Axie in Androgen-Dependent Proliferation of Prostate Cancer Cells
Abstract
The purpose of this study is to understand the role of the pl6 growth control axis in androgen dependent proliferation of prostate cancer cells. The pl6 axis contains two tumor supressors (pl6Ink4a and pRB), cyclin D-dependent kinases, and transcription factor E2F. Ne hypothesized that functions of the pl6 axis can influence androgen-dependence of prostate cancer cells. To test this hypothesis, we proposed to use controlled expression techniques to determine whether disruption of p16 axis function can lead to androgen- independence in human androgen-dependent prostate cancer cells (LNCaP) and, on the other hand, whether restoration of p16 axis function can restore androgen-dependence in androgen-independent prostate cancer cells (DU-145) . During the first year of this study, we have established conditions to establish LNCaP derivative cell lines with inducible expression of exogenously introduced proteins. For DU-145 cells we have found an essential role of pRB in maintaining androgen receptor expression. This finding may provide a missing link between androgen receptor signalling and the tumor suppression function of the p16 axis. We have established pRB inducible expression lines from DU-145 to determine the molecular mechanisms of this function of pRB.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2001
- Accession Number
- ADA397994
Entities
People
- Liang Zhu
Organizations
- Albert Einstein College of Medicine