NF1 Tumor Specific Therapeutic Modeling
Abstract
The goal of this project is to establish an animal model of plexiform neurofibroma which then can be used to test emerging anticancer therapies for the management of tumor growth in NFl patients. The first specific aim is to establish a syngraft model of NF 1 tumorigenesis. Progress toward this aim includes: a) established and characterized numerous Schwann cell cultures from Nfl knockout mouse embryos; b) effectively transfected Nf1 Schwann cell cultures with AAV-GFP; c) implanted GFP-labeled Nf1 cells in mouse nerve and detected them with GFP immunohisto-chemistry and; d) obtained evidence that transfected Nf1 cells are immuno-incompatible with wild- type host mice and adopted a new syngraft strategy using Nf1/scid mice. The second aim is to establish a xenograft model to examine the growth of human neurofibroma tumor cells. Progress toward this aim includes: a) established a breeding colony of Nfl scid mice to be used as hosts for tumor grafts; b) achieved sizeable tumors by engrafting human NF 1 Schwann cells in mouse nerves; c) published a thorough characterization of human NF 1 Schwann cells cultures and their tumorigenic properties; d) advanced MRI methods resulting in improved nerve imaging and; e) established and applied methods to assess tumorigenic growth and neovascularization by engrafted NFl tumor cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2001
- Accession Number
- ADA398060
Entities
People
- David F. Muir
Organizations
- University of Florida