The Advantages of Multi-Epitope Tumor Antigens as an Approach to Treating Breast Cancer

Abstract

Dendritic cells (DC) are an integral part of the immune system's response to cancer. A number of clinical trials have been initiated to use these cells in the treatment of melanoma, prostate cancer, and lymphoma. In preparation for treating breast cancer patients with DC, this proposal examines a fundamental issue that needs to be resolved before proceeding with this exciting new therapy. We hypothesized that the processing and presentation of multiple tumor antigen epitopes by DC is a more efficient and effective way of stimulating T cell responses than current HLA-restricted peptide-based methods. The goal of this proposal is to develop practical methods by which immune cells from patients with breast cancer can be used to promote effective anti-tumor responses. In the past year, we have continued evaluation of patient DC, developed methodology for cross- presentation of multiple tumor antigens by apoptotic tumor cell-loaded DC, completed development of assays to assess anti-tumor reactivity, and initiated experiments to compare the various antigen-arming methods. This progress is consistent with the proposal's Statement of Work, and leaves us well-positioned to complete the next goals of the proposal.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2001
Accession Number
ADA398108

Entities

People

  • Sylvia M. Kiertscher

Organizations

  • University of California, Los Angeles

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Biological Staining And Labeling
  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Culture Techniques
  • Cytokines
  • Lymphocytes
  • Molecules
  • Mononuclear Phagocyte System
  • Neoplasms
  • Production
  • Prostate Cancer
  • Reactivities
  • Test And Evaluation

Fields of Study

  • Biology
  • Medicine

Readers

  • Immunology
  • Oncology
  • Oncology (Cancer Research).