Regulation of the Response to Radiotherapy and Hyperthermia in Prostate Cancer by the 26s Proteasome
Abstract
The goal of this proposal was to quantify the extent to which human prostate cancer cells vary in their proteolytic proteasome associated activities, and to evaluate the role of the proteasome in the response of prostate cancer cells to hyperthermia and/or radiotherapy. Cell lines have been shown to vary considerably in their proteasome activity. Both irradiation and hyperthermia inhibit activity, but by different mechanisms. Further support has been obtained for the hypothesis that hyperthermia acts through HSP90 activation. We have shown that 26S, but not 20S activity, is affected by heat, while both are affected by irradiation. New techniques have been developed to look at this further.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2001
- Accession Number
- ADA398111
Entities
People
- William H. Mcbride
Organizations
- University of California, Los Angeles