Prostate Cancer Prevention Through Induction of Phase 2 Enzymes
Abstract
Virtually all human prostate cancers lose expression of glutathione S-transferase-n, an enzyme that protects against oxidative electrophiles that attack the DNA and result in cancer. Based on this observation, we proposed identifying compounds effective at inducing other carcinogen defense (phase 2) enzymes. During our Phase I Award, we identified sulforaphane as the most potent inducer of carcinogen defenses in the prostate cell. We have characterized global effects of sulforaphane in prostate cancer cell lines using cDNA microarray technology that allows large-scale determination of changes in gene expression. Our finding has been substantiated by recent epidemiologic findings of a link between high consumption of broccoli (a rich source of sulforaphane) and decreased risk of prostate cancer. These findings argue strongly for a preventive intervention trial involving supplementation with sulforaphane. Furthermore, our work demonstrates the utility of cDNA microarray technology in understanding the mechanisms of action of preventive agents. We plan to exploit this opportunity in future investigations.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2001
- Accession Number
- ADA398147
Entities
People
- James D Brooks
Organizations
- Stanford University