In Vivo Structure-Function Studies on the Precise Role of ErbB Signaling in Mammary Gland Development
Abstract
The ErbB-2 receptor tyrosine kinase is an important member for the Epidermal Growth Factor Receptor family. It has both a potent tyrosine kinase domain that can transphosphorylatate its dimerization partner or other substrates and it has a carboxy- terminal domain containing several tyrosine autophosphorylations sites. We are interested in studying the role and significance of these two domains using a physiologically relevant in vivo system. We have used a targeted knock-in strategy to generate mice expressing these mutant ErbB-2 receptors under the control of the endogenous erbB-2 promoter. The ErbB-2 kinase domain is absolutely essential for the receptors function since impairment of its activity resulted in a null phenotype. We have also generated mice expressing the ErbB-2 receptor where the five tyrosine phosphorylation sites have been mutated to phenylalanine residues (Neu-NYPD) . Characterization of these mice will begin as we expand the colony.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2001
- Accession Number
- ADA398156
Entities
People
- Richard Chan
Organizations
- McMaster University