Regulation of ErbB Receptor Tyrosine Kinase Activities in Breast Cancer by the Kek Proteins

Abstract

We have found that the Drosophila transmembrane molecule kekkon-1 (kek1) acts in a negative feed back loop to modulate the activity of the Epidermal Growth Factor Receptor (EGFR) tyrosine kinase. kek1 is expressed in response to activation of the Gurken/EGFR signaling pathway during oogenesis. While loss of kek1 activity is associated with a phenotype reminiscent of increased Grk/EGFR signaling, ectopic overexpression of kek1 mimics the complete loss of EGFR activity. We found that the extracellular domain of Kek1 physically associates with the EGFR providing the basis for this inhibitory mechanism. Interestingly, we found that Kek1 is also a potent inhibitor of the EGFR in mammalian cells.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2001
Accession Number
ADA398163

Entities

People

  • Norbert Perrimon

Organizations

  • Harvard Medical School

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cells
  • Chemistry
  • Diptera
  • Drosophila
  • Genes
  • Growth Factors
  • Kinases
  • Mammary Glands
  • Molecules
  • Neoplasms
  • Proteins
  • Tyrosine

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Electrochemical Surface Science