Investigation of the Causes of Breast Cancer at the Cellular Level: Isolation of In Vivo Binding Sites of the Human Origin Recognition Complex
Abstract
Our goal is to understand the process of DNA replication at the molecular level in human cells. We are specifically trying to identify human replicator sequences and to characterize the pre-replicative complexes at the origins of replication. In this report we summarize a series of experiments using chromatin immunoprecipitation techniques and discuss the technical problems associated with them. Then we evaluate the stability of the human origin recognition complex across the cell cycle and present a novel regulation of its large subunit.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2001
- Accession Number
- ADA398164
Entities
People
- Bruce Stillman
- Juan Mendez