The Functional Role(s) of Neurofibromin During Neural Crest Cell Development
Abstract
The purpose of the studies to date have been to A) produce and maintain a colony of heterozygote neurofibromin (Nf(sup +/-) in order to produce timed-pregnant neurofibromin knockout (Nf(sup -/-)) embryos. (B) assess the ability of in vitro and in situ studies in order to quantitate the survival, proliferation, migration and differentiation of neural crest cells derived from wild type (Nf(sup +/+)), Nf(sup +/-) and Nf(sup -/-) embryos. To achieve the aim in A, I have mated Nf(sup +/-) male mice with wild type mice to generate a colony of Nf(sup +/-) mice. This has proven more effective than directly mating male/female Nf(sup +/-) mice because Nf(sup +/-) have a greater tendency to cannibalize their litters versus wild-type females, With regards to B) I have firstly found it necessary to pair breeding mice during the day during a precise time window in order to accurately obtain embryonic day 9 (EB) time pregnant embryos. This is due to the narrow/transient time window of early NCC development in mice. Secondly, preliminary experiments strongly suggest that it is not feasible to the analysis neural crest development derived from individual embryos using a tissue culture paradigm but rather to use a in system whereby the NCC are analyzed directly in the embryo (in situ).
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2001
- Accession Number
- ADA398169
Entities
People
- Peter G. Bannerman
Organizations
- Children's Hospital of Philadelphia