Effects of c-Myc and TGF-Alpha on Polarized Membrane Traffic
Abstract
MDCK cells transformed by oncogenic Ras and Raf exhibit loss of apical polarity, cell multilayering, and alterations in the actin cytoskeleton. In response to Raf activation, these changes are preceded by the induction of Rnd3, an endogenous inhibitor of Rho protein function. The biological effects of Raf and induction of Rnd3 were prevented by pharmacological inhibitors of MEK. Expression of Rnd3 at levels equivalent to those observed following Raf transformation led to a loss of actin stress fibers. Moreover, cells co-expressing activated RhoA and Raf failed to multilayer. These data are consistent with a role for Rnd3 downstream of Raf in epithelial oncogenesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2000
- Accession Number
- ADA398203
Entities
People
- Keith Mostov
- Yoram Altschuler
Organizations
- University of California, San Francisco